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human lung microvascular endothelial cells hmvec-l  (Lonza)


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    Lonza human lung microvascular endothelial cells hmvec-l
    Human Lung Microvascular Endothelial Cells Hmvec L, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+microvascular+endothelial+cells+(hmvec-l)/human+microvascular+endothelial+cells/pm40507840-207-0-17
    Average 90 stars, based on 1 article reviews
    human lung microvascular endothelial cells hmvec-l - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Virological characteristics of a SARS-CoV-2-related bat coronavirus, BANAL-20-236.
    Article Snippet: Paired-end 76-bp sequencing was performed using a MiSeq system (Illumina) with MiSeq reagent kit v3 (Illumina, Cat# MS-102-3001).

    Article Title: Virological characteristics of a SARS-CoV-2-related bat coronavirus, BANAL-20-236
    Article Snippet: Airway-on-a-chip ( d and e) was prepared as previously described., , , , Human lung microvascular endothelial cells (HMVEC-L) were obtained from Lonza (Cat# CC-2527) and cultured with EGM-2-MV medium (Lonza, Cat# CC-3202).

    Article Title: The Development of 3D Primary Co-Culture Models of the Human Airway.
    Article Snippet: Human Lung microvascular endothelial cells (hMVEC-L) and human umbilical vein endothelial cells (HUVECs) were both obtained from Lonza (Table 2).

    Live Cell Imaging:

    Article Title: Circulating small extracellular vesicles mediate vascular hyperpermeability in diabetes
    Article Snippet: Image analysis was performed using a custom MATLAB single particle-tracking algorithm as previously reported [12]. .. Human Lung Microvascular Endothelial Cells (HMVEC-L, Lonza, Basel, CH) at 100% confluency were treated with 30 μl sEVs (in 1000 μl media) for one hour placed in a live cell imaging chamber and incubated with 10 μg/ml-1 of AF488-Albumin (Thermo Fisher Scientific, Waltham, MA, USA) in cold HPMI media (Thermo Fisher Scientific, Waltham, MA, USA) for 10 minutes at 4°C to allow apical membrane-binding. ..

    Incubation:

    Article Title: Circulating small extracellular vesicles mediate vascular hyperpermeability in diabetes
    Article Snippet: Image analysis was performed using a custom MATLAB single particle-tracking algorithm as previously reported [12]. .. Human Lung Microvascular Endothelial Cells (HMVEC-L, Lonza, Basel, CH) at 100% confluency were treated with 30 μl sEVs (in 1000 μl media) for one hour placed in a live cell imaging chamber and incubated with 10 μg/ml-1 of AF488-Albumin (Thermo Fisher Scientific, Waltham, MA, USA) in cold HPMI media (Thermo Fisher Scientific, Waltham, MA, USA) for 10 minutes at 4°C to allow apical membrane-binding. ..

    Membrane:

    Article Title: Circulating small extracellular vesicles mediate vascular hyperpermeability in diabetes
    Article Snippet: Image analysis was performed using a custom MATLAB single particle-tracking algorithm as previously reported [12]. .. Human Lung Microvascular Endothelial Cells (HMVEC-L, Lonza, Basel, CH) at 100% confluency were treated with 30 μl sEVs (in 1000 μl media) for one hour placed in a live cell imaging chamber and incubated with 10 μg/ml-1 of AF488-Albumin (Thermo Fisher Scientific, Waltham, MA, USA) in cold HPMI media (Thermo Fisher Scientific, Waltham, MA, USA) for 10 minutes at 4°C to allow apical membrane-binding. ..

    Cell Culture:

    Article Title: Human lung vascular endothelium may limit viral replication and recover in time upon the infection with rhinovirus HRV16.
    Article Snippet: Vascular endothelium is a semi-permeable barrier that regulates the flow of nutrients, ions, cytokines and immune cells between blood and tissues.. Barrier properties of endothelium, its ability to regenerate and the potential for secretion of inflammatory mediators play a crucial role in maintaining local tissue homeostasis.. The lung vascular endothelial cells were shown to be infected by human rhinovirus (HRV) and generate antiviral, inflammatory and cytopathic responses.



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    High-content imaging workflow . a ) Sample preparation. Human lung primary <t>microvascular</t> <t>endothelial</t> cells <t>(HMVEC-L)</t> and fibroblasts (IMR-90) were induced to senescence using ionizing radiation (IR). IR and mock-irradiated cells (CTL) were either cultured in full-serum medium the entire time (FS) or switched to low-serum medium for the last 3 days of culture to induce quiescence in CTL cells (SS). b ) Staining for senescence markers. Prepared samples were either co-stained for senescence-associated beta- galactosidase activity (SA-β-Gal) and proliferation via EdU incorporation (EdU); or for other senescence markers (γH2AX, LaminB1, HMGB1, p21) using immunocytochemistry (ICC). c ) High-content image analysis was performed to identify senescent subpopulations.
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    High-content imaging workflow . a ) Sample preparation. Human lung primary <t>microvascular</t> <t>endothelial</t> cells <t>(HMVEC-L)</t> and fibroblasts (IMR-90) were induced to senescence using ionizing radiation (IR). IR and mock-irradiated cells (CTL) were either cultured in full-serum medium the entire time (FS) or switched to low-serum medium for the last 3 days of culture to induce quiescence in CTL cells (SS). b ) Staining for senescence markers. Prepared samples were either co-stained for senescence-associated beta- galactosidase activity (SA-β-Gal) and proliferation via EdU incorporation (EdU); or for other senescence markers (γH2AX, LaminB1, HMGB1, p21) using immunocytochemistry (ICC). c ) High-content image analysis was performed to identify senescent subpopulations.
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    Image Search Results


    High-content imaging workflow . a ) Sample preparation. Human lung primary microvascular endothelial cells (HMVEC-L) and fibroblasts (IMR-90) were induced to senescence using ionizing radiation (IR). IR and mock-irradiated cells (CTL) were either cultured in full-serum medium the entire time (FS) or switched to low-serum medium for the last 3 days of culture to induce quiescence in CTL cells (SS). b ) Staining for senescence markers. Prepared samples were either co-stained for senescence-associated beta- galactosidase activity (SA-β-Gal) and proliferation via EdU incorporation (EdU); or for other senescence markers (γH2AX, LaminB1, HMGB1, p21) using immunocytochemistry (ICC). c ) High-content image analysis was performed to identify senescent subpopulations.

    Journal: bioRxiv

    Article Title: Senescent cell heterogeneity and responses to senolytic treatment are related to cell cycle status during cell growth arrest

    doi: 10.1101/2024.06.22.600200

    Figure Lengend Snippet: High-content imaging workflow . a ) Sample preparation. Human lung primary microvascular endothelial cells (HMVEC-L) and fibroblasts (IMR-90) were induced to senescence using ionizing radiation (IR). IR and mock-irradiated cells (CTL) were either cultured in full-serum medium the entire time (FS) or switched to low-serum medium for the last 3 days of culture to induce quiescence in CTL cells (SS). b ) Staining for senescence markers. Prepared samples were either co-stained for senescence-associated beta- galactosidase activity (SA-β-Gal) and proliferation via EdU incorporation (EdU); or for other senescence markers (γH2AX, LaminB1, HMGB1, p21) using immunocytochemistry (ICC). c ) High-content image analysis was performed to identify senescent subpopulations.

    Article Snippet: Primary human lung microvascular endothelial cells (HMVEC-L) were purchased from Lonza (CC-2527).

    Techniques: Imaging, Sample Prep, Irradiation, Cell Culture, Staining, Activity Assay, Immunocytochemistry

    Validation of senescence induction and population-level heterogeneity . a ) Representative images of senescence marker staining from full-serum (FS) samples. Top: mock-irradiated cells (CTL); bottom: ionizing-radiation-induced senescent cells (IR). For each co-staining, endothelial cells (HMVEC-L) are shown on the left; fibroblasts (IMR-90) are shown on the right. b-d ) Image quantification of HMVEC-L and IMR-90 cells for both FS and serum- starved (SS) conditions. CTL samples are in green, while IR samples are in purple. Data shown are from 2 independent experiments. b ) SA-β-Gal (left) and EdU (right) staining quantification. Each data point corresponds to one well (n = 18); bars indicate mean values. c ) Immunocytochemistry staining quantification. Top-left: γH2AX; top-right: p21; bottom-left: LaminB1; bottom-right: HMGB1. Each data point corresponds to one well (γH2AX n = 27; p21, LaminB1, and HMGB1 n = 9); bars indicate mean values. d ) Nuclear morphology feature quantification. Left: nuclear area; right: shape factor. Each data point corresponds to one well (n = 27); bars indicate mean values. ***: p-value < 10 -3 ; ****: p-value < 10 -4 ; non-significant values (p-value > 0.05) are shown.

    Journal: bioRxiv

    Article Title: Senescent cell heterogeneity and responses to senolytic treatment are related to cell cycle status during cell growth arrest

    doi: 10.1101/2024.06.22.600200

    Figure Lengend Snippet: Validation of senescence induction and population-level heterogeneity . a ) Representative images of senescence marker staining from full-serum (FS) samples. Top: mock-irradiated cells (CTL); bottom: ionizing-radiation-induced senescent cells (IR). For each co-staining, endothelial cells (HMVEC-L) are shown on the left; fibroblasts (IMR-90) are shown on the right. b-d ) Image quantification of HMVEC-L and IMR-90 cells for both FS and serum- starved (SS) conditions. CTL samples are in green, while IR samples are in purple. Data shown are from 2 independent experiments. b ) SA-β-Gal (left) and EdU (right) staining quantification. Each data point corresponds to one well (n = 18); bars indicate mean values. c ) Immunocytochemistry staining quantification. Top-left: γH2AX; top-right: p21; bottom-left: LaminB1; bottom-right: HMGB1. Each data point corresponds to one well (γH2AX n = 27; p21, LaminB1, and HMGB1 n = 9); bars indicate mean values. d ) Nuclear morphology feature quantification. Left: nuclear area; right: shape factor. Each data point corresponds to one well (n = 27); bars indicate mean values. ***: p-value < 10 -3 ; ****: p-value < 10 -4 ; non-significant values (p-value > 0.05) are shown.

    Article Snippet: Primary human lung microvascular endothelial cells (HMVEC-L) were purchased from Lonza (CC-2527).

    Techniques: Marker, Staining, Irradiation, Immunocytochemistry